Effects of Aqueous extract of Garlic (Allium Sativa L) on Alcohol-Duodenal ulcers of Adult Wistar Rats: a Histological Study

Abstract

Background:Garlic (Allium sativa L) is a species in the onion family Alliaceae. The aim of the present study is to investigate the histological effects of aqueous extract of garlic on alcohol-induced duodenal ulcers of adult wistar rats. Methods: Adult wistar rats of both sexes (n=30) with weight between 150250g were randomly selected into three treatment groups (B, C, D); a positive control (A), and a negative control (E) groups of five rats each. The treatment groups were fasted overnight before being induced with alcohol to cause duodenal toxicity, after which each group (B, C, D) respectively received daily administration of 200, 400 and 600mg/kg of body weight of garlic extracts (GE) for 21 days through orogastric tube, with the confirmation of duodenal injury. The rats were subjected to light anesthesia (Chloroform) in a urethane-saturated chamber and sacrificed at the 22nd day of the experiment, and duodenums were carefully dissected and quickly fixed in 10% formal saline for histological study. Results: At the end of a 3 weeks treatment period, wide range of mucosal injury and ulcer was observed. However, when alcohol treated rats were given aqueous extract of garlic, the mucosal injury and ulcer was significantly reduced in all organs to nearly those of control rats. Conclusion: Garlic (Allium sativa) extract is able to inhibit the alcohol-induced duodenal toxicity