Assessment of Bacterial Profile of Pus Samples Obtained From Patients Undergoing Surgical Procedures: An Observational Study

Bacterial Profile of Pus Samples

Abstract

Background: Wound infection may initially be manifest as bacterial colonization, and it is only when colonization is combined with other factors, such as decreased vascular supply, intrinsic virulence of specific bacteria (eg, Staphylococcus aureus), and host immune factors, that true infection occurs. Hence; we planned the present study to assess bacterial profile of pus samples obtained from patients undergoing surgical procedures. Subjects and Methods: The present study included assessment of 30 pus samples sent from the surgery department, to the microbiology department. Collection of pus samples was done from the infected wounds. Detailed demographic data of all the patients was obtained. In the microbiology department, for studying the morphological profile of the clinical isolates, gram staining was done. Streaking of the pus samples was done on the blood agar, and MacConkey agar followed on incubation aerobically at 37 degree centigrade for 24 hour. Identification of microorganisms was done based on colonial morphology and pigment production. All the results were compiled and analysed by SPSS software. Results: Staphylococcus aureus was the most commonly isolated bacteria in the present study. Other bacterias obtained in the present study included Pseudomonas aeruginosa, Klebsiella species, Escherichia coli, Enterococci species and Enterobacter species. Conclusion: Most common organism isolated from pus discharge from infected wounds is S. aureus.