Determination of Role of Hyaluronan in Recurrent Renal Stone Formation

Hyaluronan & renal stone formation

Abstract

Background: Extracorporeal shock wave lithotripsy (ESWL) and percutaneous nephrolithotomy (PCNL) are now widely used to eradicate stones. The present study was conducted to determine role of hyaluronan in recurrent renal stone formation. Subjects and Methods: The study was conducted at Department of Urology, Sapthagiri Institute of Medical Sciences and Research Center, Bangalore between November 2019  to August 2020 on 45 patients of both genders were classified into 3 groups. Group I were normal (15), group II (15) were stone formers and group III (15) were post- treated stone formers. The total urinary glycosaminoglycans, hyaluronan and the proportion of HA in total GAGs were recorded. Results: There were 8 males and 7 females in group I, 6 males and 9 females in group II and 10 males and 5 females in group III. The total urinary glycosaminoglycans (GAGs) concentration in group I was 235.4 g hexuronate/ mmol creatinine, in group II was 142.3 g hexuronate/ mmol creatinine and in group III was 108.2 g hexuronate/ mmol creatinine. The mean hyaluronan level in group I was 812.4 g hexuronate/ mmol creatinine, in group II was 1725.4 g hexuronate/ mmol creatinine and in group III was 672.4 g hexuronate/ mmol creatinine. The mean HA in total GAGs was 0.26% in group I, 0.74% in group II and 1.04% in group III. The difference was significant (P< 0.05). Conclusion: Increased HA production during inflammation of renal epithelial cells in SF do enhance the risk of renal stone formation and an higher HA proportion in total GAGs of both SF and Post-SF indicated that they have a higher risk for the occurrence and recurrence of kidney stone disease.