Evaluation of the Diagnostic Value of Mycobacterium Tuberculosis DNA Recognition by Polymerase Chain Reaction (PCR) on Bone Tuberculosis

Diagnostic Value of Mycobacterium Tuberculosis

  • Varun Singh Assistant Professor, Department of Orthopaedics, Saraswathi Institute of Medical Sciences, NH-9, Anwarpur, Pillkhuwa, Hapur, Uttar Pradesh, India
  • Shubhendu Gupta Associate Professor, Department of T.B. & Chest, Saraswathi Institute of Medical Sciences, NH-9, Anwarpur, Pillkhuwa, Hapur, Uttar Pradesh, India
Keywords: Polymerase Chain Reaction, Mycobacterium Tuberculosis, DNA, ELISA

Abstract

Background: This study aimed at evaluating the function of polymerase chain reaction (PCR) in the identification of spinal tuberculosis post two weeks of preoperative anti-tuberculosis cure and to evaluate PCR to the Lowenstein - Jensen Culture (LJC) and histopathological examination (HPE) methods. Subjects and Methods: All of the tissues obtained for histopathology were fixed in 10% formalin, embedded in paraffin, cut to 5-µm-thick sections and stained with hematoxylin-eosin (H&E) and Gabbet’s stain before microscopic examination. The sample collection was executed in sterile situations. Joint fluid, pus or tissue samples were composed and ground. Results: Results demonstrated that the PCR approach had 80.9% sensitivity, 95.2% specificity and 87.3% accuracy respectively. ELISA for TB-SA antibody had 51.8% sensitivity, 82.4% specificity and 66.4% accuracy respectively. The bacterial culture had 80.9% sensitivity, 68.1% specificity and 43.2% accuracy respectively. Conclusion : Polymerase chain reaction (PCR) is a speedy technique for identifying extrapulmonary TB with high sensitivity and specificity.

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Published
2020-12-30
How to Cite
Singh, V., & Gupta, S. (2020). Evaluation of the Diagnostic Value of Mycobacterium Tuberculosis DNA Recognition by Polymerase Chain Reaction (PCR) on Bone Tuberculosis. Asian Journal of Medical Research, 9(4), 11-15. Retrieved from http://aijournals.com/index.php/ajmr/article/view/1857