Evaluation of the Diagnostic Value of Mycobacterium Tuberculosis DNA Recognition by Polymerase Chain Reaction (PCR) on Bone Tuberculosis

Diagnostic Value of Mycobacterium Tuberculosis

  • Varun Singh Assistant Professor, Department of Orthopaedics, Saraswathi Institute of Medical Sciences, NH-9, Anwarpur, Pillkhuwa, Hapur, Uttar Pradesh, India
  • Shubhendu Gupta Associate Professor, Department of T.B. & Chest, Saraswathi Institute of Medical Sciences, NH-9, Anwarpur, Pillkhuwa, Hapur, Uttar Pradesh, India
Keywords: Polymerase Chain Reaction, Mycobacterium Tuberculosis, DNA, ELISA

Abstract

Background: This study aimed at evaluating the function of polymerase chain reaction (PCR) in the identification of spinal tuberculosis post two weeks of preoperative anti-tuberculosis cure and to evaluate PCR to the Lowenstein - Jensen Culture (LJC) and histopathological examination (HPE) methods. Subjects and Methods: All of the tissues obtained for histopathology were fixed in 10% formalin, embedded in paraffin, cut to 5-µm-thick sections and stained with hematoxylin-eosin (H&E) and Gabbet’s stain before microscopic examination. The sample collection was executed in sterile situations. Joint fluid, pus or tissue samples were composed and ground. Results: Results demonstrated that the PCR approach had 80.9% sensitivity, 95.2% specificity and 87.3% accuracy respectively. ELISA for TB-SA antibody had 51.8% sensitivity, 82.4% specificity and 66.4% accuracy respectively. The bacterial culture had 80.9% sensitivity, 68.1% specificity and 43.2% accuracy respectively. Conclusion : Polymerase chain reaction (PCR) is a speedy technique for identifying extrapulmonary TB with high sensitivity and specificity.

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References

Butt T, Karamat KA, Ahmad RN, Mahmood A. Advances in diagnosis of tuberculosis. Pak J Pathol. 2001;12(1):1–3.

Manganelli R, Dubnau E, Tyagi S, Kramer FR, Smith I. Differential expression of 10 sigma factor genes in Mycobacterium tuberculosis. Mol Microbiol. 1999;31(2):715– 724. Available from: https://dx.doi.org/10.1046/j.1365-2958.1999.01212.x.

Soini H, Musser JM. Molecular Diagnosis of Mycobacteria. Clin Chem. 2001;47(5):809–814. Available from: https://dx.doi.org/10.1093/clinchem/47.5.809.

Styrt BA, Shinnick TM, Ridderhof JC, Crawford JT, Tenover FC. Turnaround times for mycobacterial cultures. J clin microbiol. 1997;35(4):1041–1042. Available from: https://dx.doi.org/10.1128/jcm.35.4.1041-1042.1997.

Brown TJ, Power EG, French GL. Evaluation of three commer- cial detection systems for Mycobacterium tuberculosis where clinical diagnosis is difficult. J Clin Pathol. 1999;52(3):193–197. Available from: https://dx.doi.org/10.1136/jcp.52.3.193.

Rajalahti I, Vuorinen P, Liippo K, Nieminen M, Miettinen A. Evaluation of Commercial DNA and rRNA Amplification Assays for Assessment of Treatment Outcome in Pulmonary Tuberculosis Patients. Eur J Clin Microbiol Infect Dis. 2001;20(10):746–750. Available from: https://dx.doi.org/10.1007/s100960100593.

Bogard M, Vincelette J, Antinozzi R, Alonso R, Fenner T, Schirm J, et al. Multicenter Study of a Commercial, Automated Polymerase Chain Reaction System for the Rapid Detection of Mycobacterium tuberculosis in Respiratory Specimens in Routine Clinical Practice. Eur J Clin Microbiol Infect Dis. 2001;20(10):724–731. Available from: https://dx.doi.org/10.1007/s100960100565.

Almeda J, Garcia A, Gonzalez J. Clinical evaluation of an in-house IS6110 polymerase chain reaction for diagnosis of tuberculosis. Eur J ClinMicrobiol Infect Dis. 2000;19:859–67. Available from: https://doi.org/10.1007/s100960000375.

Yuen KY, Chan KS, Chan CM, Ho BS, Dai LK, Chau PY, et al. Use of PCR in routine diagnosis of treated and untreated pulmonary tuberculosis. J Clin Pathol. 1993;46(4):318–322. Available from: https://dx.doi.org/10.1136/jcp.46.4.318.

Bang D, Andersen SR, e EV, Rasmussen EM. Performance of the GenoType MTBDRplus assay (v2.0) and a new extended GenoType MTBDRsl assay (v2.0) for the molecular detection of multi- and extensively drug-resistant Mycobacterium tuber- culosis on isolates primarily from Lithuania. Diagn Micro- biol Infect Dis. 2016;86(4):377–381. Available from: https://dx.doi.org/10.1016/j.diagmicrobio.2016.08.026.

Speth MT, Repnik U, Griffiths G. Layer-by-layer nanocoating of live Bacille-Calmette-Guérin mycobacteria with poly(I:C) and chitosan enhances pro-inflammatory activation and bac- tericidal capacity in murine macrophages. Biomaterials. 2016;111:1–12. Available from: https://dx.doi.org/10.1016/j.biomaterials.2016.09.027.

Ozdemir ZM, Kahraman AS, Gormeli CA, Sevimli R, Akpolat N. Langerhans Cell Histiocytosis with Atypical Intervertebral Disc and Sacroiliac Joint Involvement Mimicking Osteoartic- ular Tuberculosis in an Adult. Balk Med J. 2016;33(5):573–577. Available from: https://dx.doi.org/10.5152/balkanmedj.2016.160492.

Guessogo WR, Mandengue SH, Ndemba PBA, Medjo UO, Minye EE, Ahmaidi S, et al. Physical and functional follow-up of tuberculosis patients in initial intensive phase of treatment in Cameroon using the 6-min walk test. J Exerc Rehabil. 2016;12(4):333–339. Available from: https://dx.doi.org/10.12965/jer.1632620.310.

Mittal S, Khalid M, Sabir AB, Khalid S. Comparison of Magnetic Resonance Imaging Findings between Pathologically Proven Cases of Atypical Tubercular Spine and Tumour Metastasis: A Retrospective Study in 40 Patients. Asian Spine J. 2016;10(4):734. Available from: https://dx.doi.org/10.4184/asj.2016.10.4.734.

Fernandes S, Vieira-Sousa E, Furtado C, Costa A, Barros R, Fonseca JE. A diagnosis of disseminated tuberculosis based on knee arthroscopic guided synovial biopsy in the context of monoarthritis. ActaReumatol Port. 2016;41:256–259.

Held M, Laubscher M, Mears S, Dix-Peek S, Workman L, Zar H, et al. Diagnostic Accuracy of the Xpert MTB/RIF Assay for Extrapulmonary Tuberculosis in Children With Musculoskeletal Infections. Pediatr Infect Dis J. 2016;35(11):1165–1168. Available from: https://dx.doi.org/10.1097/inf.0000000000001271.

Mihailovic J, Matovina E, Nikoletic K. 18F- fluorideoxyglucose positron emission tomogra- phy/computed tomography imaging: Artifacts and pit- falls. Med Pregl. 2015;68(1-2):41–48. Available from: https://doi.org/10.2298/mpns1502041m.

Li W, Xiao DM, Jiang CQ, Zhang WT, Lei M. Arthroscopic treatment of bony loose bodies in the subacromial space. Int J Surg Case Rep. 2015;11:101–104. Available from: https://doi.org/10.1016/j.ijscr.2015.02.004.

Tuli SM, Srivastava TP, Varma BP, Sinha GP. Tuberculosis of Spine. Acta Orthop Scand. 1967;38(1-4):445–458. Available from: https://dx.doi.org/10.3109/17453676708989653.

Vohra R, Kang HS, Dogra S, Saggar RR, Sharma R. TUBERCULOUS OSTEOMYELITIS. J Bone Joint Surg Br. 1997;79-B(4):562–566. Available from: https://dx.doi.org/10.1302/0301-620x.79b4.0790562.

Grosskopf I, David B, Charach A, Hochman G, I. Bone and joint tuberculosis-a 10-year review. Isr J Med Sci. 1994;30:278–283.

Martini M, Adjrad A, Boudjemaa A. Tuberculous osteomyeli- tis. A review of 125 cases. Int Orthop. 1986;10:201–207. Avail- able from: https://doi.org/10.1007/bf00266209.

der Spoel van Dijk AV, McLeod A, Botha PL, Shipley JA, Kapnoudhis MA, Beukes CA. The diagnosis of skeletal tuberculosis by polymerase chain reaction. Cent Afr J Med. 2000;46(6):144–149. Available from: https://dx.doi.org/10.4314/cajm.v46i6.8546.

Negi SS, Gupta S, Khare S, Lal S. Comparison of various microbiological tests including polymerase chain reaction for the diagnosis of osteoarticular tuberculosis. Indian J Med Microbiol. 2005;23:245–248.

Jou NT, Yoshimori RB, Mason GR, Louie JS, Liebling MR. Single-tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis. J clin Microbiol. 1997;35(5):1161–1165. Available from: https://dx.doi.org/10.1128/jcm.35.5.1161-1165.1997.

Muniyandi M, Ramachandran R, Balasubramanian R, Narayan PR. Socio-economic dimensions of tuberculosis control: review of studies over two decades from Tuberculosis Research Center. J Commun Dis. 2006;38(3):204–215.

Rajeswari R, Muniyandi M, Balasubramanian R, Narayanan PR. Perceptions of tuberculosis patients about their physical, mental and social well-being: a field report from south India. Soc Sci Med. 2005;60(8):1845–1853. Available from: https://dx.doi.org/10.1016/j.socscimed.2004.08.024.

Published
2020-12-30
How to Cite
Singh, V., & Gupta, S. (2020). Evaluation of the Diagnostic Value of Mycobacterium Tuberculosis DNA Recognition by Polymerase Chain Reaction (PCR) on Bone Tuberculosis. Asian Journal of Medical Research, 9(4), 11-15. Retrieved from https://aijournals.com/index.php/ajmr/article/view/1857