A Study on Prevalence of Serum Resistant & Sensitive Escherichia Coli in Urinary Tract Infection

Prevalence of Escherichia Coli in Urinary Tract Infection

  • K. Prakash Associate Professor, Department of Microbiology, CAIMS, Karimnagar, Telangana, India
  • J. Shravan Kumar Assistant Professor, Department of Surgery, Prathima Institute of Medical Sciences, Karimnagar, Telangana, India
  • J. Rajamouli Professor & HOD, Department of Microbiology, CAIMS, Karimnagar, Telangana, India
  • Sachin Gurnule Statistician Cum Lecturer, Department of Microbiology, CAIMS, Karimnagar, Telangana, India
Keywords: Uropathogenic, Resistant, Sensitivity

Abstract

Background: Escherichia coli is the commonest organism causing urinary tract infection in women and children especially in those with uncom- plicated infections. It has been traditionally described Serotypes of Escherichia coli were consistently associated with Uropathogenicity and designated as Uropathogenic Escherichia coli.It is now recognized that a subset of fecal Escherichia coli can be colonized in the peri-urethral area, enter the urinary tract and cause symptomatic disease. These are currently defined as Uropathogenic Escherichia coli. The aims & objectives are to the isolation of Escherichia coli in Urinary tract infection. 2. To determine the prevalence of Serum resistant Uropathogenic Escherichia coli. 3. To compare antimicrobial efficacy in Serum resistant and Serum sensitive Uropathogenic Escherichia coli. Subjects & Methods: An observational study was conducted on 100 patients and urine samples collected from suspected and suffering from urinary tract infection. Study period from August 2018 to July 20019 attending General medicine OP in Hospital. Escherichia coli were isolated and identified by conventional techniques Mackie & Mc Cartney. The strains were subjected to multiple drugs to study serum resistance and sensitivity. Results: From 100 urine samples 71 Uropathogenic, 18 males & 53 females. 40% Sero-resistant and 60% Sero-sensitive among them 2 to 18 years males and > 40 years females resistant, above 40 years males and 19 to 40 years females are sensitive to multiple antibiotics. Conclusion: it emphasizes Serum resistance is an important virulence factor, It may lead to pyelonephritis and septicemia. Serum resistant E. coli are multiple drug-resistant, so the present study formulating guidelines for planning effective treatment and Periodic surveillance to monitor resistance.

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References

Kausar Y, Sneha K, Chunchanur, Shobha D, Nadagir L, Halesh MR, et al. Virulence factors, sero types and antimicrobial susceptibility pattern of Escherichia coli in Urinary tract infections. Al Ameen J Med Sci. 2009;2(1):47–51.

Raksha R, Srinivasa H, Macaden RS. Occurrence and characterisation of Uropathogenic Escherichia coli in urinary tract infections. Indian J Med Microbiol. 2003;21(2):102–107.

Bhat GK, Mallya S, Hegde A. Effect of exposure to hydrogen peroxide on the virulence of Escherichia coli. Indian J Med Microbiol. 2008;26(1):25–25. Available from: https://dx.doi.org/10.4103/0255-0857.38853.

Siegfried L, Kmetova M, Janigova V, Sasinka M, Takacova V. Serum Response of Escherichia coli Strains causing Dyspepsia and Urinary tract infection: Relation to Alpha_hemolysin production and O Type. Infect Immun. 1963;11:4543–4545.

Bhat GK, Shenoy S, Sharma S. Virulence factors and drug resistance in Escherichia coli isolated from extraintesti- nal infections. Indian J Med Microbiol. 2007;25(4):369. Avail- able from: https://dx.doi.org/10.4103/0255-0857.37341.

Taylor PW, Parton R. A Protein Factor Associated With Serum Resistance in Escherichia Coli. J Med Microbiol. 1977;10(2):225–232. Available from: https://dx.doi.org/10.1099/00222615-10-2-225.

Kot B. Antibiotic Resistance Among Uropathogenic Escherichia coli. Pol J Microbiol. 2019;68(4):403–415. Available from: https://dx.doi.org/10.33073/pjm-2019-048.

Roberts A, Philips R. The relative importance of the classical and alternative complement pathways in Serum bacterial activity against Escherichia coli. Journal of Medical Microbiology. 1983;(16):69–74.

Siegfried L, Puzova H, Kmetcova M, Kerstesova A. Killing of α-haemolytic and non-haemolytic Escherichia coli strains in human serum and polymorphonuclear leucocytes. J Med Microbiol. 1992;37(1):3–7. Available from: https://dx.doi.org/10.1099/00222615-37-1-3.

Bugla-Płoskońska G, Cisowska A, Karpińska K, Jankowski S, Doroszkiewicz W. The mechanisms of activation of normal human serum complement byEscherichia coli strains with K1 surface antigen. Folia Microbiol. 2006;51(6):627–632. Available from: https://dx.doi.org/10.1007/bf02931630.

Primack W, Bukowski T, Sutherland R, Gravens-Mueller L, Carpenter M. What Urinary Colony Count Indicates a Urinary Tract Infection in children? J Pediatr. 2017;191:259–61. Available from: https://dx.doi.org/10.1016/j.jpeds.2017.08.012.

Iwase T, Tajima A, Sugimoto S, ichi Okuda K, Hironaka I, Kamata Y, et al. A Simple Assay for Measuring Catalase Activity: A Visual Approach. Sci Rep. 2013;3:3081. Available from: https://dx.doi.org/10.1038/srep03081.

Ma M, Amano T, Enokimoto M, Yano T, Moe KK, Misawa N. Influence of pH of TSI medium on the detection of hydrogen sulfide production by Campylobacter hyointestinalis. Lett Appl Microbiol. 2007;44(5):544–9. Available from: https://dx.doi.org/10.1111/j.1472-765X.2006.02097.x.

Jones RN, Glick T, Sader HS, Flamm RK, Ross JE, Rhomberg PR, et al. Educational antimicrobial susceptibility testing as a critical component of microbiology laboratory proficiency pro- grams: American Proficiency Institute results for 2007-2011. Diagn Microbiol Infect Dis . 2013;75(4):357–60. Available from: https://dx.doi.org/10.1016/j.diagmicrobio.2013.01.027.

Syal K, Mo M, Yu H, Iriya R, Jing W, Guodong S, et al. Current and emerging techniques for antibiotic susceptibility tests. Theranostics. 2017;7(7):1795–1805. Available from: https://dx.doi.org/10.7150/thno.19217.

Published
2020-12-30
How to Cite
K. Prakash, J. Shravan Kumar, J. Rajamouli, & Sachin Gurnule. (2020). A Study on Prevalence of Serum Resistant & Sensitive Escherichia Coli in Urinary Tract Infection. Asian Journal of Medical Research, 9(4), 1-6. https://doi.org/10.47009/ajmr.2020.9.4.MB1